CRISPR

CRISPR1-cyp11a1.2

ID

ZDB-CRISPR-181129-5

Name

CRISPR1-cyp11a1.2

Previous Names

CRISPR1-cyp11a2

Target

cyp11a1.2 (1)

Sequence

5' - GGAGTCTGTCGGCCCGTC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ihb317	cyp11a1.2
ihb318	cyp11a1.2

Expression

Gene expression in Wild Types + CRISPR1-cyp11a1.2

No data available

Phenotype

Phenotype resulting from CRISPR1-cyp11a1.2

No data available

Phenotype of all Fish created by or utilizing CRISPR1-cyp11a1.2

Phenotype	Fish	Conditions	Figures
whole organism 11-oxotestosterone decreased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 2 from Wang et al., 2021
ovary casp9 expression increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 5 from Wang et al., 2021
testis apoptotic process increased occurrence, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 5 from Wang et al., 2021
testis hypertrophic, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 2 from Wang et al., 2021
Sertoli cell gsdf expression increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
Leydig cell cyp11c1 expression increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
male gonad development increased occurrence, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 5 from Wang et al., 2021
spermatid differentiation disrupted, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 3 from Wang et al., 2021
whole organism estradiol decreased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 2 from Wang et al., 2021
testis hsd3b1 expression increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
whole organism cortisol decreased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 2 from Wang et al., 2021
whole organism 11-Ketoandrosterone decreased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 2 from Wang et al., 2021
testis nanos2 expression increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 6 from Wang et al., 2021
oogenesis arrested, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 4 from Wang et al., 2021
urogenital papilla protruding, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 2 from Wang et al., 2021
spermatocyte increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 3 from Wang et al., 2021
testis cyp19a1a expression increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
sperm amount, ameliorated	cyp11a1.2^{ihb317/ihb317} (AB)	chemical treatment by environment: pregnenolone	Fig. 5 from Wang et al., 2021
anal fin light yellow, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 2 from Wang et al., 2021
testis cyp11a1.2 expression decreased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 1 from Wang et al., 2021
testis gdf9 expression increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
Leydig cell insl3 expression decreased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
testis inha expression decreased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
testis bmp15 expression increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
female gonad development decreased occurrence, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 5 from Wang et al., 2021
whole organism male with DSD, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 2 from Wang et al., 2021
Sertoli cell amh expression increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
ovary tp53 expression increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 5 from Wang et al., 2021
spermatogenic cyst decreased area, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 5 from Wang et al., 2021
testis sycp3 expression decreased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 6 from Wang et al., 2021
spermatogonium increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 3, Fig. 6 from Wang et al., 2021
testis igf3 expression decreased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
female sex determination absent process, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 2, Fig. 4 from Wang et al., 2021
gonad increased weight, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 2 from Wang et al., 2021
testis ddx4 expression increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 6 from Wang et al., 2021
whole organism progesterone decreased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 2 from Wang et al., 2021
sperm decreased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 3, Fig. 5 from Wang et al., 2021
spermatogenic cyst area, ameliorated	cyp11a1.2^{ihb317/ihb317} (AB)	chemical treatment by environment: pregnenolone	Fig. 5 from Wang et al., 2021
testis cyp17a1 expression increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
testis sox9b expression increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
testis star expression increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
female gonad development normal occurrence, ameliorated	cyp11a1.2^{ihb317/ihb317} (AB)	chemical treatment by environment: pregnenolone	Fig. 5 from Wang et al., 2021
testis cell diploid, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 3 from Wang et al., 2021
female gonad development decreased occurrence, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	chemical treatment by environment: estradiol	Fig. 5 from Wang et al., 2021
testis gsdf expression increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
testis cimap1b expression decreased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 6 from Wang et al., 2021
male gonad development increased occurrence, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	chemical treatment by environment: estradiol	Fig. 5 from Wang et al., 2021
male organism decreased fecundity, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 3 from Wang et al., 2021
testis insl3 expression decreased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
whole organism testosterone decreased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 2 from Wang et al., 2021
spermatid decreased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 3 from Wang et al., 2021
male gonad development normal occurrence, ameliorated	cyp11a1.2^{ihb317/ihb317} (AB)	chemical treatment by environment: pregnenolone	Fig. 5 from Wang et al., 2021
testis cell population proliferation increased occurrence, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 6 from Wang et al., 2021
Sertoli cell inha expression absent, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
spermatogenesis disrupted, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 3 from Wang et al., 2021
testis cyp11c1 expression increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
testis amh expression increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
Leydig cell cyp17a1 expression increased amount, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 7 from Wang et al., 2021
testis cyp11a1.2 expression absent, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 1 from Wang et al., 2021
whole organism androst-4-ene-3,17-dione absent, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 2 from Wang et al., 2021
male mating behavior disrupted, abnormal	cyp11a1.2^{ihb317/ihb317} (AB)	standard conditions	Fig. 3 from Wang et al., 2021

Citations