CRISPR

CRISPR1-abcb11b

ID

ZDB-CRISPR-181003-1

Name

CRISPR1-abcb11b

Previous Names

CRISPRS1-abcb11b

Target

abcb11b (1)

Sequence

5' - GGAGCAGGGGTCTTCATTCT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ci200	abcb11b

Expression

Gene expression in Wild Types + CRISPR1-abcb11b

No data available

Phenotype

Phenotype resulting from CRISPR1-abcb11b

No data available

Phenotype of all Fish created by or utilizing CRISPR1-abcb11b

Phenotype	Fish	Conditions	Figures
bile acid secretion decreased occurrence, abnormal	abcb11b^ci200/ci200	chemical treatment by environment: chloroquine	Fig. 6 from Ellis et al., 2017
hepatocyte mitochondrion malformed, abnormal	abcb11b^ci200/ci200	standard conditions	Fig. 3 from Ellis et al., 2017
bile canaliculus abcb4 expression spatial pattern, abnormal	abcb11b^ci200/ci200	standard conditions	Fig. 5 from Ellis et al., 2017
bile canaliculus increased width, abnormal	abcb11b^ci200/ci200	standard conditions	Fig. 3 from Ellis et al., 2017
hepatocyte glycogen decreased amount, abnormal	abcb11b^ci200/ci200	standard conditions	Fig. 3 from Ellis et al., 2017
hepatocyte cytoplasm abcb4 expression mislocalised, abnormal	abcb11b^ci200/ci200	standard conditions	Fig. 5 from Ellis et al., 2017
hepatocyte swollen, abnormal	abcb11b^ci200/ci200	standard conditions	Fig. 3 from Ellis et al., 2017
hepatocyte mitochondrial crista degenerate, abnormal	abcb11b^ci200/ci200	standard conditions	Fig. 3 from Ellis et al., 2017
bile canaliculus decreased length, abnormal	abcb11b^ci200/ci200	standard conditions	Fig. 3 from Ellis et al., 2017
bile acid secretion decreased occurrence, abnormal	abcb11b^ci200/ci200	standard conditions	Fig. 4, Fig. 6 from Ellis et al., 2017
liver becn1 expression increased amount, abnormal	abcb11b^ci200/ci200	standard conditions	Fig. 6 from Ellis et al., 2017
hepatocyte has extra parts of type hepatocyte autophagosome, abnormal	abcb11b^ci200/ci200	standard conditions	Fig. 6 from Ellis et al., 2017
hepatocyte intracellular canaliculus abcb4 expression spatial pattern, abnormal	abcb11b^ci200/ci200	standard conditions	Fig. 5 from Ellis et al., 2017
bile canaliculus malformed, abnormal	abcb11b^ci200/ci200	standard conditions	Fig. 3 from Ellis et al., 2017
hepatocyte necrotic, abnormal	abcb11b^ci200/ci200	standard conditions	Fig. 3 from Ellis et al., 2017
hepatocyte autophagy increased occurrence, abnormal	abcb11b^ci200/ci200	standard conditions	Fig. 6 from Ellis et al., 2017
hepatocyte plasma membrane degenerate, abnormal	abcb11b^ci200/ci200	standard conditions	Fig. 3 from Ellis et al., 2017
hepatocyte mitochondrion swollen, abnormal	abcb11b^ci200/ci200	standard conditions	Fig. 3 from Ellis et al., 2017
bile acid secretion occurrence, ameliorated	abcb11b^ci200/ci200	chemical treatment by environment: sirolimus	Fig. 6 from Ellis et al., 2017
bile canaliculus decreased length, abnormal	abcb11b^ci200/+	standard conditions	Fig. 3 from Ellis et al., 2017
bile acid secretion decreased occurrence, abnormal	abcb11b^ci200/+	chemical treatment by environment: chloroquine	Fig. 6 from Ellis et al., 2017
hepatocyte apical plasma membrane abcb11b expression spatial pattern, ameliorated	abcb11b^ci200/ci200; um14Tg	chemical treatment by environment: sirolimus	Fig. 6 from Ellis et al., 2017
hepatocyte intracellular canaliculus abcb11b expression spatial pattern, ameliorated	abcb11b^ci200/ci200; um14Tg	chemical treatment by environment: sirolimus	Fig. 6 from Ellis et al., 2017
bile canaliculus abcb11b expression position, ameliorated	abcb11b^ci200/ci200; um14Tg	chemical treatment by environment: sirolimus	Fig. 6 from Ellis et al., 2017
hepatocyte intracellular canaliculus abcb11b expression absent, abnormal	abcb11b^ci200/ci200; um14Tg	standard conditions	Fig. 2 from Ellis et al., 2017
hepatocyte abcb11b expression mislocalised, abnormal	abcb11b^ci200/ci200; um14Tg	control	Fig. 6 from Ellis et al., 2017
bile canaliculus abcb11b expression absent, abnormal	abcb11b^ci200/ci200; um14Tg	standard conditions	Fig. 2 from Ellis et al., 2017
bile acid secretion decreased occurrence, ameliorated	abcb11b^ci200/ci200; ci202Tg	standard conditions	Fig. 4 from Ellis et al., 2017

Citations