CRISPR

CRISPR2-mir223

ID

ZDB-CRISPR-180706-2

Name

CRISPR2-mir223

Previous Names

None

Target

mir223 (1)

Sequence

5' - TTTGTCAAATACCCCAAGAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
pu18	mir223

Expression

Gene expression in Wild Types + CRISPR2-mir223

No data available

Phenotype

Phenotype resulting from CRISPR2-mir223

No data available

Phenotype of all Fish created by or utilizing CRISPR2-mir223

Phenotype	Fish	Conditions	Figures
whole organism cul1a expression increased amount, abnormal	mir223^pu18/pu18	transection: caudal fin	Fig. 3 from Zhou et al., 2018
neutrophil chemotaxis process efficacy, ameliorated	mir223^pu18/pu18	transection: caudal fin, chemical treatment: NF-kappaB inhibitor	Fig. 3 from Zhou et al., 2018
whole organism traf6 expression increased amount, abnormal	mir223^pu18/pu18	transection: caudal fin	Fig. 3 from Zhou et al., 2018
neutrophil chemotaxis increased efficacy, abnormal	mir223^pu18/pu18	transection: caudal fin	Fig. 1 , Fig. 2 , Fig. 3 from Zhou et al., 2018
caudal fin neutrophil increased amount, exacerbated	mir223^pu18/pu18	transection: caudal fin	Fig. 1 , Fig. 2 , Fig. 3 from Zhou et al., 2018
fin regeneration increased efficacy, abnormal	mir223^pu18/pu18	transection: caudal fin	Fig. 6 from Zhou et al., 2018
caudal fin neutrophil increased amount, ameliorated	mir223^pu18/pu18	transection: caudal fin, chemical treatment: NF-kappaB inhibitor	Fig. 3 from Zhou et al., 2018
regenerating fin increased length, abnormal	mir223^pu18/pu18	transection: caudal fin	Fig. 6 from Zhou et al., 2018
caudal fin canonical NF-kappaB signal transduction increased process quality, abnormal	mir223^pu18/pu18; nc1Tg	control	Fig. 4 from Zhou et al., 2018
caudal fin EGFP expression increased amount, abnormal	mir223^pu18/pu18; nc1Tg	control	Fig. 4 from Zhou et al., 2018
caudal fin canonical NF-kappaB signal transduction increased process quality, abnormal	mir223^pu18/pu18; nc1Tg	transection: caudal fin	Fig. 4 from Zhou et al., 2018
caudal fin EGFP expression increased amount, abnormal	mir223^pu18/pu18; nc1Tg	transection: caudal fin	Fig. 4 from Zhou et al., 2018
neutrophil chemotaxis process efficacy, ameliorated	mir223^pu18/pu18 + CRISPR7-myd88 + CRISPR8-myd88	transection: caudal fin	Fig. 3 from Zhou et al., 2018
caudal fin neutrophil increased amount, ameliorated	mir223^pu18/pu18 + CRISPR7-myd88 + CRISPR8-myd88	transection: caudal fin	Fig. 3 from Zhou et al., 2018
caudal fin neutrophil increased amount, ameliorated	mir223^pu18/pu18 + CRISPR1-cul1a + CRISPR1-cul1b + CRISPR2-cul1a + CRISPR2-cul1b	transection: caudal fin	Fig. 3 from Zhou et al., 2018
neutrophil chemotaxis process efficacy, ameliorated	mir223^pu18/pu18 + CRISPR1-cul1a + CRISPR1-cul1b + CRISPR2-cul1a + CRISPR2-cul1b	transection: caudal fin	Fig. 3 from Zhou et al., 2018
caudal fin neutrophil increased amount, ameliorated	mir223^pu18/pu18; pu9Tg	transection: caudal fin	Fig. 2 from Zhou et al., 2018
neutrophil chemotaxis process efficacy, ameliorated	mir223^pu18/pu18; pu9Tg	transection: caudal fin	Fig. 2 from Zhou et al., 2018
caudal fin EGFP expression amount, ameliorated	mir223^pu18/pu18; nc1Tg + MO1-rac2 + MO1-spi1b	transection: caudal fin	Fig. 4 from Zhou et al., 2018
caudal fin canonical NF-kappaB signal transduction process quality, ameliorated	mir223^pu18/pu18; nc1Tg + MO1-rac2 + MO1-spi1b	transection: caudal fin	Fig. 4 from Zhou et al., 2018

Citations