CRISPR

CRISPR1-otx2b

ID

ZDB-CRISPR-180208-1

Name

CRISPR1-otx2b

Previous Names

None

Target

Sequence

5' - GGAACCCGGCTAATTGTCTC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

This CRISPR was designed to target approximately 500 base pairs upstream of the ATG start site.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Expression

Gene expression in Wild Types + CRISPR1-otx2b

No data available

Phenotype

Phenotype resulting from CRISPR1-otx2b

No data available

Phenotype of all Fish created by or utilizing CRISPR1-otx2b

Phenotype	Fish	Conditions	Figures
midbrain otx2b expression decreased amount, abnormal	otx2b^{tud44a/tud44a}; otx2b^{tud44Tg/tud44Tg} (AB)	control	Fig. 2 from Kesavan et al., 2018
eye pigmentation decreased occurrence, abnormal	otx2b^tud44Tg; tud118Tg	heat shock, chemical treatment by environment: tamoxifen	Fig. 5 from Hans et al., 2021
midbrain cell adhesion decreased efficacy, abnormal	otx2b^tud40Tg + MO1-cdh2	chemical treatment by environment: ethylene glycol bis(2-aminoethyl)tetraacetic acid	Fig. 5 from Kesavan et al., 2020
midbrain cell adhesion decreased efficacy, abnormal	otx2b^tud40Tg + MO1-cdh2	standard conditions	Fig. 5 from Kesavan et al., 2020
hindbrain cell adhesion decreased efficacy, abnormal	otx2b^tud40Tg + MO1-cdh2	chemical treatment by environment: ethylene glycol bis(2-aminoethyl)tetraacetic acid	Fig. 5 from Kesavan et al., 2020

Citations