MGC:109977

ID

ZDB-CDNA-050522-146

Name

MGC:109977

Symbol

MGC:109977

Previous Names

• FR_8027 (1)

Type

cDNA

Location

Unmapped

Genome Resources

None

Species

Danio rerio

Library

NIH_ZGC_9

Digest

Insert Size

1300

Cloning Site

EcoRI-PstI

Vector Type

Plasmid

Vector

pME18S-FL3

Polymerase

T3 RNA polymerase

PCR Amplification

Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGTACGGAAGTGTTACTTCTGCTC3'
5'GGATCCATTAACCCTCACTAAAGGGAAGGCCGCGACCTGCAGCTC3'

Suppliers

None

Quality

( Simple to use, intense expression pattern restricted to a few structures )

Protocol

Thisse in situ hybridization protocol

Note

None