MGC:91966

ID

ZDB-CDNA-040704-2

Name

MGC:91966

Symbol

MGC:91966

Previous Names

• FR_7199 (1)

Type

cDNA

Location

Unmapped

Genome Resources

None

Clone Problem Type

NONSENSE_MEDIATED_DECAY__NMD_

Species

Danio rerio

Library

NIH_ZGC_10

Digest

Insert Size

2360

Cloning Site

NotI-SmaI

Vector Type

Plasmid

Vector

pExpress-1

Polymerase

T7 RNA polymerase

PCR Amplification

Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TAATACGACTCACTATAGGG 3'
5' GGATCCATTAACCCTCACTAAAGGGAAGTTTCGCTCCGGAATTCCCGGG 3'

Suppliers

None

Quality

( Nice strong expression pattern )

Protocol

Thisse in situ hybridization protocol

Note

None