cDNA

MGC:85750

ID
ZDB-CDNA-040516-16
Name
MGC:85750
Symbol
MGC:85750
Previous Names
  • FR_6958 (1)
Type
cDNA
Location
Unmapped
Genome Resources
None
Species
Danio rerio
Library
NCI_CGAP_ZKid1
Digest
Insert Size
1768
Cloning Site
NotI-SmaI
Vector Type
phagemid
Vector
pCMV-SPORT6.1
Polymerase
T7 RNA polymerase
PCR Amplification
Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' AACAGCTATGACCATGATTAC 3'
5' GTAAAACGACGGCCAGT 3'
Suppliers
None
Quality
( Probe is difficult to use. Generally basal level of expression with more intense labeling in particular structure. )
Protocol
Thisse in situ hybridization protocol
Note
None
Gene Expression
Directly Submitted Expression Data
1 figure (1 image) from Thisse et al., 2004 [MGC:85750]
Marker Relationships
Sequences
Citations