MGC:56252

ID

ZDB-CDNA-040425-3658

Name

MGC:56252

Symbol

MGC:56252

Previous Names

• FR_4826 (1)

Type

cDNA

Location

Chr: 21 Mapping Details/Browsers

Genome Resources

None

Species

Danio rerio

Library

Sugano SJD adult male

Digest

Insert Size

1967

Cloning Site

EcoRI-PstI

Vector Type

Plasmid

Vector

pME18S-FL3

Polymerase

T3 RNA polymerase

PCR Amplification

Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGTACGGAAGTGTTACTTCTGCTC3'
5'GGATCCATTAACCCTCACTAAAGGGAAGGCCGCGACCTGCAGCTC3'

Suppliers

None

Quality

( Probe is difficult to use. Generally basal level of expression with more intense labeling in particular structure. )

Note

None