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Fig. 4 AMBRA1 morphant embryos display increased cell proliferation. (A) Representative images of phospho-histone H3 immunohistochemistry in WT and AMBRA1 morphant embryos at 9 hpf. Scale bar, 200 μm. (B) The numbers of proliferating cells were counted and compared between WT embryos, AMBRA1 morphant embryos, and AMBRA1 morphant embryos co-injected with hAMBRA1 or mutated hAMBRA1PXP mRNAs. For each condition, 10 embryos were analyzed, and the experiment was performed twice. Statistical analysis was performed using Student's t-test. *p < 0.05; ***p < 0.001; ****p < 0.0001. Color images are available online.