FIGURE

Fig 8

ID

ZDB-FIG-230823-13

Publication

Islam et al., 2023 - The RNA-binding protein Adad1 is necessary for germ cell maintenance and meiosis in zebrafish

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Fig 8

Apoptosis and proliferation are not affected in adad1 mutants.

A-F: Cell death assays in 60 dpf adad1^Y67X wild-type and mutant testes. Neither TUNEL assays (A,B,E) nor cleaved-Caspase 3 immunofluorescence (B,D,F) showed a significant difference between wild-type and mutant testes. Arrowheads indicate examples of TUNEL and cleaved-Caspase3 positive cells. G-P: Cell proliferation assay in 45 dpf testes using BrdU labeling. Fish were treated overnight with BrdU then waited for 6 hours without BrdU prior to fixation (G). Immunostaining was done with BrdU, Ddx4/Vasa, and Sycp3 antibodies to detect proliferating spermatogonia. Spermatogonia were identified as Ddx4/Vasa positive and Sycp3 negative (arrowheads, H-O). Quantification of the percentage of BrdU positive spermatogonia showed no significant difference between wild-types and mutants (P). SG = spermatogonia. Scale bar: 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	adad1^{sa14397/sa14397}
Observed In:	testis apoptotic process testis cell population proliferation
Stage:	Days 45-89

Phenotype Detail

Acknowledgments

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