FIGURE

Figure 7

ID
ZDB-FIG-230523-58
Publication
Oprişoreanu et al., 2023 - Drug screening in zebrafish larvae reveals inflammation-related modulators of secondary damage after spinal cord injury in mice
Other Figures
All Figure Page
Back to All Figure Page
Figure 7

Treatment with Cimetidine reduces secondary damage induced by spinal cord contusion injury in mice. A: Confocal images of the spinal cord at 28-day survival after injury stained with anti-GFAP are shown. Note that the size and extent of the lesion is smaller in the Cimetidine treated group compared to the vehicle-treated control (quantified in C). B: Luxol fast blue (LFB) stained sections of the spinal cord 28 days after SCI. Note greater sparing of myelin LFB staining in the Cimetidine-treated group (quantified in D). C: Quantification of tissue loss is shown. Note although the mean values in the Cimetidine treated group are smaller than in the vehicle-treated group, the value reaches statistical significance only at - 500 µm rostral to the lesion due to the variability between animals (Two-way RM-ANOVA; group effect F(1,8) = 3.188, p = 0.1120; with Sidak's multiple comparison test; p*(-500) = 0.0372, n = 5 mice per group. Note also that the longitudinal extent of the lesion is smaller in the Cimetidine-treated group. D: Mice treated with Cimetidine show a significant reduction of myelin loss (LFB staining) from the center of the lesion to 600 μm rostrally (Two-way RM-ANOVA; group effect F(1,7) = 12.35, p = 0.0098; with post-hoc Bonferroni multiple comparison test; p*(-600µm) = 0.0438, p*(-400µm) = 0.0204, p**(-200µm) = 0.0084, p*(center) = 0.0103, n = 4-5 mice per group). Scale bars = 200 µm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Theranostics