Figure 1

High expression of mitochondrial organization‐related genes in juvenile ovary. A) K‐means clustering of high‐variation genes in gonads. The genes of cluster 5 were highly expressed in the juvenile ovaries at 25 and 30 dpf. B) GO enrichment analysis of cluster 5 genes. C) Heatmap of mitochondrial organization‐related genes expression in juvenile ovary and testis at 25 and 30 dpf. D) Heatmap of oxidative phosphorylation‐related genes expression in juvenile ovary and testis at 25 and 30 dpf. E) K‐means clustering of high‐variation genes in trunks. The expression of cluster 4 genes was lower in dnd‐MO than in wildtype at 14 and 22 dpf. F) Heatmap of highly expressed genes overlapped between clusters 5 and 4 from gonads differentiating toward ovary. G) Detection of tissue‐specific gene expression by RT‐PCR. H) Detection of PGC‐specific gene expression by WISH. Scale bar: 200 µm for left; 100 µm for right. N represents analyzed embryo number. I) RT‐qPCR verification of differential expression of pld6, org, zgc:103482, and rnf17 in juvenile ovary and testis of wildtype. Every three gonads were mixed into a sample and three biological replicates were performed. J) RT‐qPCR verification of differential expression of pld6, org, zgc:103482, and rnf17 in gonads of wildtype and dnd‐MO. Every three gonads were mixed into a sample and three biological replicates were performed. The data were expressed as mean ± SD. The P values in this figure were calculated by two‐sided t‐test. *P < 0.05; **P < 0.01; ***P < 0.001; MO, morpholino; WISH, whole‐mount in situ hybridization; PGC, primordial germ cell; dpf, days post fertilization; RT‐qPCR, reverse‐transcription quantitative PCR.