FIGURE

Figure 5

ID
ZDB-FIG-221211-182
Publication
Drummond et al., 2022 - osr1 Maintains Renal Progenitors and Regulates Podocyte Development by Promoting wnt2ba via the Antagonism of hand2
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Figure 5

Figure 5. wnt2ba is sufficient for podocyte development downstream of osr1. (A) When wnt2ba was assessed at 15 ss, in ocn−/− and WT siblings, it was evident that staining was reduced in mutants. By 24 hpf, wnt2ba staining was almost completely absent, tracking with the loss of other podocyte markers in ocn−/−. Scale bar is 30 μm. (B) FISH experiments showed that osr1 and wnt2ba colocalized in cells at 22 ss. (CH) Embryos were injected with osr1 MO and/or wnt2ba cRNA at the one-cell stage, and podocytes and the developing slit diaphragm were visualized at 24 hpf using wt1b and nphs1, respectively. Embryos injected with osr1 MO alone showed few podocyte or slit diaphragm cells, while embryos injected with wnt2ba cRNA alone had an increased podocyte area. Coinjected embryos had a partial rescue of podocytes, indicating that wnt2ba is a downstream factor in the podocyte pathway. A minimum of 5 individuals were imaged for quantification. p-values: ** p < 0.001, * p < 0.05, N.S. = not significant. Scale bar is 30 μm.

Expression Data
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 14-19 somites to Prim-5

Phenotype Detail
Acknowledgments
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