FIGURE

FIGURE 3

ID
ZDB-FIG-211002-13
Publication
Peña et al., 2021 - Differential Requirement of Gata2a and Gata2b for Primitive and Definitive Myeloid Development in Zebrafish
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FIGURE 3

Gata2b is necessary for HSPC specification but dispensable for HSPC maturation. (A–C) Analysis of runx1 expression at 36 hpf by in situ hybridization in Gata2bu5008 mutant embryos shows decreased expression in gata2bu5008/u5008 homozygotes. (D) Quantification of runx1 expression in the AGM of 36 hpf mutant embryos shows allele dependent decrease of runx1 expression. (E) Number of itga2b:GFPlo cells by flow cytometry of individual Tg(itga2b:GFP) Gata2bu5008 mutant embryos at 3 dpf. (F–H) Expression of c-myb by in situ hybridization in the CHT of 4 dpf mutant larvae. (I) Quantification of c-myb+ cells in the CHT of 4 dpf mutant fish. In (J–L), lateral views of 48 hpf mutant embryos stained with SB, labeling granulocytes. (M) Quantification of SB+ cells in the CHT of 48 hpf mutant embryos. In (N–P), SB stainings of 4 dpf larvae show allele dependent decrease of SB+ cells in the CHT of Gata2bu5008 mutant fish, quantified in (Q).

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to Day 4

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Prim-25 to Day 4

Phenotype Detail
Acknowledgments
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