FIGURE

Fig. 4

ID
ZDB-FIG-210825-13
Publication
Luo et al., 2021 - Cooperation between liver-specific mutations of pten and tp53 genetically induces hepatocarcinogenesis in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

Mutation of tp53 is critical for the progression of hepatocarcinogenesis. a Abundances of Ptena, Ptenb, or Fabp10 mRNA in liver tissues of fabp10WT and pten;tp53 cKO fish (n = 3 per group). b Western blot analysis of Fabp10, Tp53, and Pten in liver tissues of WT, fabp10WT, and pten;tp53 cKO fish (n = 3 per group). c Overall survival rates of WT, fabp10WT, and pten;tp53 cKO fish (n = 200 per group). d Gross morphology of 3-month-old fabp10WT (di) and pten;tp53 cKO fish (dvi) and 6-month-old pten;tp53 cKO fish (dxi). Representative bright field images of the internal abdominal organs, with the liver outlined, in 3-month-old fabp10WT (dii) and pten;tp53 cKO fish (dvii) and 6-month-old pten;tp53 cKO fish (dxii). in, intestine; li, liver tissues, sb, swim bladder. Histological examination of liver tissues from fabp10WT and pten;tp53 cKO fish at 3 and 6 months of age, respectively. Several typical hepatocarcinogenesis phenotypes were observed in 3- and 6-month-old pten;tp53 cKO fish, including abnormal lipid accumulation in hepatocytes (black arrows, dviii), bile duct disappearance (green arrowheads, dviii-x), vascular disorder (black arrowheads, d9), variation in nuclear/cellular sizes (pleomorphism; dvii, viii), and tumour cell invasion into blood vessels (dxiii), the pancreas (dxiv), and the kidney (dxv). Scale bars, 100 μm. e Immunohistochemical staining was performed to examine the expression of Gs, Pcna, Ck19, and pAkt in liver tissues from 3- and 6-month-old fabp10WT and pten;tp53 cKO fish. Scale bars, 100 μm. f Western blot analyses were performed to confirm the expression of Pten, Tp53, pAkt, and Akt in liver tissues from 3-month-old fabp10WT (lanes 1–2) and pten;tp53 cKO fish (lanes 3–4). g Quantitative analysis of the protein expression ratio of pAkt and Akt in liver tissues from 3-month-old fabp10WT and pten;tp53 cKO fish. The data are shown as the mean ± SEM values. *p < 0.05, ***p < 0.001

Expression Data
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Days 30-44 to Adult

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Days 30-44 to Adult

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Exp. Clin. Cancer Res.