Fig. 5.

Dysmorphic bone geometry in crocc2 mutants. A geometric morphometric shape analysis was performed on various element of the feeding apparatus in WT and crocc2 mutant fish. Mutants exhibit distinct mandible shapes compared to WT siblings, with the most conspicuous differences occurring in the size and shape of the coronoid process (B vs. A). Scale bars in (A) and (B) equal 1 mm. Shape differences were also noted for the kinethmoid, with mutants exhibiting an overall shortening of the element in the dorsal–ventral dimension (D vs. C). Scale bars in (C) and (D) equal 200 µm. Deformation grids represent commonly seen phenotypes in the mandible, and exaggerated mean shapes in the kinethmoid. Procrustes ANOVA with post-hoc pairwise comparisons of group means (procD.lm, advanced.procD.lm), was significant for mandible mean shapes at P = 0.02, and for kinethmoid means at P = 0.12.