Fig. 6

Synergistic effect of zebrafish Mci and E2f5 in inducing ectopic MCCs and MCC-specific genes. (A) Short primary cilia are present in the myotome of wild-type embryos at 24 hpf (uninjected, lateral view of the somites). While injection of zebrafish mci, e2f4 or e2f5 RNAs alone did not produce any ectopic cilia, co-injection of the mci and e2f5 RNAs (mci ​+ ​e2f5), but not co-injection of the mci and e2f4 RNAs (mci ​+ ​e2f4), resulted in the formation of ectopic multiple cilia in the myotome of the majority of samples examined. Images taken are representative of the 2 independent experiments performed (n ​= ​15 each). Asterisk marks a nerve branch that is also labeled with the anti-acetylated tubuin antibodies. Scale bar ​= ​5 ​μm. (B–D) qPCRs were performed on uninjected 24 hpf embryos or embryos injected with mci (A), e2f5 (B), or mci and e2f5 (C) mRNAs, respectively. Expression levels in the uninjected conditions were arbitrarily assigned a value of 1. The rplpo gene was used as an internal (loading) control. Logarithmic scale was used for the Y-axes. Error bars represent SD from 3 independent experiments.