FIGURE

Fig. 3

ID

ZDB-FIG-190301-19

Publication

Yoo et al., 2017 - Sinup is essential for the integrity of centrosomes and mitotic spindles in zebrafish embryos

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Effect of misforced expression of sinup on spindle organization. (A) sinup-EGFP-injected embryo. (B) sinup144A-EGFP-injected embryo. The embryos were scanned at the sphere stage with a confocal microscope. The embryos injected with sinup144A-EGFP showed disruption in the mitotic spindle organization, orientation, and polarity of the spindle pole. Multipolar spindles were labeled with white star. (C) In vitro phosphorylation assay of Sinup144A recombinant protein. Phosphorylation of Sinup144A by Aurora A was greatly reduced in comparison with that of wild-type Sinup. (D) Purified recombinant proteins were stained with Coomassie brilliant blue on SDS-PAGE gel. 1: Marker (Fermentas #SM0671), 2: GST protein, 3: Sinup-GST, 4: Sinup144A-GST.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Animal Cells Syst (Seoul)