FIGURE

Fig. 3

ID

ZDB-FIG-180103-26

Publication

Lu et al., 2017 - Ablation of EYS in zebrafish causes mislocalisation of outer segment proteins, F-actin disruption and cone-rod dystrophy

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

The thickness of ONL decreased and the apoptosis of retinal cells increased in the EYS^−/− zebrafish.

(A) Retinal histology analysis of WT and EYS^−/− zebrafish. Retinal sections of EYS^−/− zebrafish stained with hematoxylin/eosin (HE) at indicated ages. RPE, retinal pigment epithelium; OS, outer segment; IS, inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bars: 50 μm. (B) TUNEL staining between WT and EYS^−/− zebrafish. Scale bars: 50 μm. (C) Comparison of the ONL thickness between WT and EYS^−/− zebrafish at indicated ages (n = 3). Quantitative analysis revealed a significant reduction of the ONL thickness at 3 mpf (P = 0.0479), 4 mpf (P = 0.0258), 8 mpf (P = 0.0122), 16 mpf (P = 0.0272) in the EYS^−/− zebrafish. (D) Quantification of TUNEL positive cells in WT and EYS^−/− zebrafish at indicated ages (n = 3). Quantitative analysis revealed a significant increase of TUNEL positive cells at 8 mpf (P = 0.0341), 16 mpf (P = 0.0028) in the EYS^−/− zebrafish.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	eys^hzu4/hzu4
Observed In:	retina apoptotic process retinal outer nuclear layer
Stage Range:	Days 30-44 to Days 45-89

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.