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Fig. 1

ID

ZDB-FIG-130228-32

Publication

Shimada et al., 2012 - A high-throughput fluorescence-based assay system for appetite-regulating gene and drug screening

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Fig. 1

Zebrafish feeding assay using 4-Di-10-ASP labeled paramecia.

(A) Schematic representation of the feeding assay. (B) The actual numbers of paramecia correlated with optical density at 500 nm (OD₅₀₀). (C) Correlation between the relative amount of paramecia (P) and fluorescent intensity. (D) Fluorescent intensities of ingested paramecia in zebrafish. The fluorescent intensities of paramecia in zebrafish abdomen increased independently to the numbers of introduced-paramecia. All values are mean ± SEM, n = 15. (E) Fluorescent intensities after free-feeding of 4-10-Di-ASP-labeled paramecia. Fluorescent intensities of ingested paramecia were at maximum levels 1-3 h after feeding. All values are mean ± SEM, n = 15, *P<0.05, **P<0.01 compared with 1 h after feeding.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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