FIGURE

Fig. 2

ID
ZDB-FIG-070822-3
Publication
Wakahara et al., 2007 - fibin, a novel secreted lateral plate mesoderm signal, is essential for pectoral fin bud initiation in zebrafish
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Fig. 2

Detection of recombinant mouse Fibin expressed in COS-7 cells. (A) COS-7 cells were transfected with the empty vector (Control) or the mouse Fibin-expression vector (Fibin). The cell lysate (a, c) and culture medium (b, d) of the transfected COS-7 cells were separated by SDS–polyacrylamide gel electrophoresis under reducing conditions and transferred onto a nitrocellulose membrane. The protein with the E tag on the membrane was detected using anti-E tag antibody. (B) COS-7 cells transfected with the empty vector (Control) (a–f) or the mouse Fibin-expression vector (Fibin) (g–l) were examined by immunohistochemistry using anti-mannosidase II antibody for the Golgi apparatus (a, d, g, j) and anti-E tag antibody for recombinant Fibin (b, e, h, k). The signals obtained by immunohistochemistry using anti-mannosidase II antibody and anti-E tag antibody were merged (c, f, i, l). Scale bar = 20 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Biology, 303(2), Wakahara, T., Kusu, N., Yamauchi, H., Kimura, I., Konishi, M., Miyake, A., and Itoh, N., fibin, a novel secreted lateral plate mesoderm signal, is essential for pectoral fin bud initiation in zebrafish, 527-535, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.