CRISPR

CRISPR1-cct4

ID

ZDB-CRISPR-180523-1

Name

CRISPR1-cct4

Previous Names

None

Target

cct4 (1)

Sequence

5' - TCGACGCTGGTCGCGGTAGC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
pc34	cct4

Expression

Gene expression in Wild Types + CRISPR1-cct4

No data available

Phenotype

Phenotype resulting from CRISPR1-cct4

No data available

Phenotype of all Fish created by or utilizing CRISPR1-cct4

Phenotype	Fish	Conditions	Figures
skeletal muscle cell Z disc Ab1-cct5 labeling decreased amount, abnormal	cct4^pc34/pc34	standard conditions	Fig. 2 from Berger et al., 2018
skeletal muscle skeletal myofibril assembly decreased occurrence, abnormal	cct4^pc34/pc34	standard conditions	Fig. 2 from Berger et al., 2018
skeletal muscle cell skeletal muscle myofibril decreased amount, abnormal	cct4^pc34/pc34	standard conditions	Fig. 2 from Berger et al., 2018
myotome skeletal muscle cell has fewer parts of type skeletal muscle cell skeletal muscle myofibril, abnormal	cct4^pc34/pc34	standard conditions	Fig. 2 from Berger et al., 2018
whole organism cct4 expression absent, abnormal	cct4^pc34/pc34	standard conditions	Fig. 2 from Berger et al., 2018
skeletal muscle cell Ab1-cct5 labeling spatial pattern, abnormal	cct4^pc34/pc34	standard conditions	Fig. 2 from Berger et al., 2018
eye morphology, abnormal	cct4^pc34/pc34 (TU)	standard conditions	Figure 1 from Berger et al., 2023
eye deformed, abnormal	cct4^pc34/pc34 (TU)	standard conditions	Figure 1 from Berger et al., 2023
lens deformed, abnormal	cct4^pc34/pc34 (TU)	standard conditions	Figure 1 from Berger et al., 2023
retina deformed, abnormal	cct4^pc34/pc34 (TU)	standard conditions	Figure 1 from Berger et al., 2023
skeletal muscle cell Z disc EGFP expression absent, abnormal	cct4^pc34/pc34; pc22Tg; pc32Tg	standard conditions	Fig. 2 from Berger et al., 2018
skeletal muscle cell EGFP expression spatial pattern, abnormal	cct4^pc34/pc34; pc22Tg; pc32Tg	standard conditions	Fig. 2 from Berger et al., 2018

Citations