Term Name: | 5-bromo-4-chloro-3-indolyl beta-D-galactoside |
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Synonyms: | 5-bromo-4-chloro-1H-indol-3-yl beta-D-galactopyranoside, 5-bromo-4-chloro-3-indolyl beta-galactoside, 5-bromo-4-chloroindol-3-yl-beta-D-galactopyranoside, BCIG, Indoxyl-Gal, X-gal |
Definition: | An indolyl carbohydrate that is the beta-D-galactoside of 3-hydroxy-1H-indole in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. It is used to test for the presence of an enzyme, beta-galactosidase, which cleaved the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole, which immediately dimerises to give an intensely blue product. |
Ontology: | ChEBI [CHEBI:75055] ( EBI ) |