Fig. 4

cdh7 expression in 12–18 hpf zebrafish embryos. All panels, except (C, G, and H), show lateral views of whole mount embryos (anterior to the left and dorsal up) labeled with either cdh7 cRNA (A–G and I) or cdh7 and pax2a cRNA probes (H, both probes were digoxigenin-labeled). (B) Higher magnification of the head region of the embryo with the eye primordium outlined by the dashed line. (C) Dorsal view (anterior to the left) of the embryo. The arrows in (B and C) point to the same cdh7 expression region in the hindbrain, while arrowheads in (C) indicate cdh7 expression at the midline. (E–G) Higher magnifications of the head region, while (I) is a higher magnification of the tail region. The dashed line in (E) indicates the optic recess, while the arrowhead points to the caudal boundary of the cerebellum. The pair of arrowheads in (F) bracket a hindbrain region with reduced cdh7 expression. (G and H) Dorsal views (anterior to the left) of higher magnifications of the hindbrain region of the embryos showing that the region with the reduced cdh7 expression is at the level of the otic placode. The otic placodes in (G) are outlined with dashed lines. Abbreviations: c, cerebellum; ey, eye premordium; h, hindbrain; he, head region; nc, notochord; op, otic placode; sp, spinal cord.