TALEN

TALEN1-esama

ID
ZDB-TALEN-170601-1
Name
TALEN1-esama
Previous Names
None
Target
Target Sequence 1
5' - GTTGCCTTATGAAAA - 3'
Target Sequence 2
5' - GCAGCACCACCATCT - 3'
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
Forward TALEN RVD (NH NG NG NH HD HD NG NG NI NG NH NI NI NI NI) Reverse TALEN RVD (NH HD NI NH HD NI HD HD NI HD HD NI NG HD NG)
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
ubs19 esama
Expression
Gene expression in Wild Types + TALEN1-esama
No data available
Phenotype
Phenotype resulting from TALEN1-esama
No data available
Phenotype of all Fish created by or utilizing TALEN1-esama
Phenotype Fish Conditions Figures
central artery blood vessel lumenization decreased process quality, abnormal esamaubs19/ubs19; ubs16Tg control Fig. S4 from Hübner et al., 2018
intersegmental vessel disconnected, abnormal esamaubs19/ubs19; y1Tg standard conditions Fig. 5 with image from Sauteur et al., 2017
intersegmental vessel disconnected, exacerbated cdh5ubs8/ubs8; esamaubs19/ubs19; y1Tg standard conditions Fig. 5 with image from Sauteur et al., 2017
intersegmental vessel tight junction ab1-tjp1 labeling spatial pattern, abnormal cdh5ubs8/ubs8; esamaubs19/ubs19; y1Tg standard conditions Fig. 6 with image from Sauteur et al., 2017
intersegmental vessel tight junction disconnected, abnormal cdh5ubs8/ubs8; esamaubs19/ubs19; y1Tg standard conditions Fig. 6 with image from Sauteur et al., 2017
intersegmental vessel EGFP expression mislocalised, abnormal cdh5ubs8/ubs8; esamaubs19/ubs19; y1Tg standard conditions Fig. 6 with image from Sauteur et al., 2017
intersegmental vessel ab1-tjp1 labeling mislocalised, abnormal cdh5ubs8/ubs8; esamaubs19/ubs19; y1Tg standard conditions Fig. 5 with image from Sauteur et al., 2017
intersegmental vessel basal plasma membrane podxl2 expression mislocalised, abnormal cdh5ubs8/ubs8; esamaubs19/ubs19; y1Tg standard conditions Fig. 6 with image from Sauteur et al., 2017
cell-cell junction maintenance disrupted, abnormal cdh5ubs8/ubs8; esamaubs19/ubs19; y1Tg standard conditions Fig. 6 with image from Sauteur et al., 2017
apical junction assembly disrupted, abnormal cdh5ubs8/ubs8; esamaubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg standard conditions Fig. 4 with image from Sauteur et al., 2017
actin filament organization disrupted, abnormal cdh5ubs8/ubs8; esamaubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg standard conditions Fig. 4 with image from Sauteur et al., 2017
actin cytoskeleton organization disrupted, abnormal cdh5ubs8/ubs8; esamaubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg standard conditions Fig. 4 with image from Sauteur et al., 2017
endothelial tip cell cell adhesion decreased efficacy, exacerbated cdh5ubs8/ubs8; esamaubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg standard conditions Fig. 3 with imageFig. 4 with image from Sauteur et al., 2017
cell adhesion involved in sprouting angiogenesis disrupted, abnormal cdh5ubs8/ubs8; esamaubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg standard conditions Fig. 4 with image from Sauteur et al., 2017
dorsal longitudinal anastomotic vessel blood vessel development delayed, abnormal cdh5ubs8/ubs8; esamaubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg standard conditions Fig. 3 with image from Sauteur et al., 2017
endothelial tip cell aggregated, abnormal cdh5ubs8/ubs8; esamaubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg standard conditions Fig. 3 with image from Sauteur et al., 2017
dorsal longitudinal anastomotic vessel blood vessel development delayed, exacerbated cdh5ubs8/ubs8; esamaubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg standard conditions Fig. 4 with image from Sauteur et al., 2017
endothelial tip cell actin filament bundle of filopodium decreased amount, abnormal cdh5ubs8/ubs8; esamaubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg standard conditions Fig. 3 with imageFig. 4 with image from Sauteur et al., 2017
angiogenic sprout has extra parts of type endothelial cell filopodium, abnormal cdh5ubs8/ubs8; esamaubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg standard conditions Fig. 3 with image from Sauteur et al., 2017
angiogenic sprout endothelial tip cell detached from angiogenic sprout, exacerbated cdh5ubs8/ubs8; esamaubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg standard conditions Fig. 3 with image from Sauteur et al., 2017
Citations