Person
Sampath, Karuna
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Biography and Research Interest
Research Interests:
Maternal Nodal and regulation of Nodal signalling
Functions and regulation of non-coding nodal RNA
RNAs with coding and non-coding roles in development (cncRNAs)
RNA transport, localization, translational control
Polarity, microtubule dynamics
Methods to interrogate functions and dynamics of RNAs and RBPs
Research Summary:
Research in the Sampath laboratory focuses on fundamental mechanisms that control development and differentiation of embryonic progenitors and stem cells, and the molecular mechanisms underlying these processes. We are investigating the roles and regulation of RNAs and RNA-binding proteins (RBPs) in vertebrates using zebrafish embryos and human cells as experimental systems. We use genetic, genomic, imaging, proteomic, and embryological approaches to focus on the following themes: 1) Roles and regulation of maternal nodal RNA as a paradigm for RNAs with both coding and non-coding functions (cncRNAs) 2) Regulation of developmental signaling by RNA elements, RBPs, and RNA regulons, following on our work on translational control of nodal/squint RNA 3) Microtubule based transport and localization of RNAs, and 4) Human fetal and maternal disorders arising from mutations in RNA control elements and RBPs.
Maternal Nodal and regulation of Nodal signalling
Functions and regulation of non-coding nodal RNA
RNAs with coding and non-coding roles in development (cncRNAs)
RNA transport, localization, translational control
Polarity, microtubule dynamics
Methods to interrogate functions and dynamics of RNAs and RBPs
Research Summary:
Research in the Sampath laboratory focuses on fundamental mechanisms that control development and differentiation of embryonic progenitors and stem cells, and the molecular mechanisms underlying these processes. We are investigating the roles and regulation of RNAs and RNA-binding proteins (RBPs) in vertebrates using zebrafish embryos and human cells as experimental systems. We use genetic, genomic, imaging, proteomic, and embryological approaches to focus on the following themes: 1) Roles and regulation of maternal nodal RNA as a paradigm for RNAs with both coding and non-coding functions (cncRNAs) 2) Regulation of developmental signaling by RNA elements, RBPs, and RNA regulons, following on our work on translational control of nodal/squint RNA 3) Microtubule based transport and localization of RNAs, and 4) Human fetal and maternal disorders arising from mutations in RNA control elements and RBPs.
Non-Zebrafish Publications
CncRNAs: RNAs with both coding and non-coding roles in development.
Sampath K1, Ephrussi A2. Development. 143(8):1234-41. doi: 10.1242/dev.133298. 2016
Keeping a lid on nodal: transcriptional and translational repression of nodal signalling.
Karuna Sampath, Elizabeth J. Robertson. Open Biol. 2016, 6 150200; DOI: 10.1098/rsob.150200.
http://rsob.royalsocietypublishing.org/content/6/1/150200
P Kumari and K Sampath. cncRNAs: Bi-functional RNAs with protein coding and non-coding functions. Seminars in Cell and Developmental Biology. doi:10.1016/j.semcdb.2015.10.024 (2015).
Gilligan, P, P Kumari, S Lim, A Cheong, A Chang and K Sampath. Conservation defines functional motifs in the squint/nodal-related 1 RNA Dorsal Localization Element. Nucleic Acids Research doi: 10.1093/nar/gkq1185 (2010).
Tao, S and K Sampath. Alternative splicing of Smads in differentiation and tissue homeostasis.
Development, Growth & Differentiation 52: 335-342 (2010).
Sampath, K., Cheng, A.M.S., Frisch, A. and Wright, C.V.E. (1997). Functional differences among Xenopus nodal-related genes in left-right axis determination. Development 124, 3293-3303.
Lowe, L.A., Supp, D.M., Sampath, K., Yokoyama, T., Wright, C.V.E., Potter, S.S., Overbeek, P. and Kuehn, M.R. (1996). Conserved left-right asymmetry of nodal expression and alterations in murine situs inversus. Nature 381, 158-161.
Stuart, G. W., Zhu, Z., Sampath, K. and King, M.W. (1995). POU-domain genes from the flatworm Dugesia tigrina. Gene 161, 299-300.