Figure 1

The family of NADPH oxidases

(A–E) All NOXes are transmembrane proteins that transport oxygen across biological membranes, reducing oxygen to superoxide, which can then undergo dismutation to generate H₂O₂. NOXes are multi-componential proteins, and p22phox (the product of the cyba gene) is a common subunit to NOX1-4. Zebrafish lack Nox3 and have a single Duox isoform. Mutants for cyba (B) and nox5 (C) were generated using CRISPR, resulting in a 5bp deletion (cyba^umc403) and a 4bp indel (nox5^umc402), respectively. The mutations led to the removal of restriction enzyme sites, providing an easy diagnostic method for screening animals. Characterization of cyba^sa11798 (D) and duox^sa9892 (E) via Sanger sequencing shows the single nucleotide changes T>A and C>T, respectively, in contrast to a WT reference sequence. Panel A courtesy Dr. Kalin Narov, https://kalinnarov.wixsite.com/embryosafari.

See also Figures S1 and S2.