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Fig. 4

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ZDB-IMAGE-220715-46
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Figures for Berger et al., 2022
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Fig. 4

Sarcomere organisation is compromised in mob4geh mutants.

(A) Transmission electron micrograph depicted highly organised and arrayed myofibrils in 3-dpf-old siblings (n = 3). (B) Organised sarcomeres were rarely detected within mob4geh homozygotes (n = 3). (B’) As shown in the magnification of the boxed area, sarcomeres were frequently disorganised and deposits of isolated filaments (asterisk) in addition to electron-dense structures (arrowhead), often associated with Z-disks, were found instead. (C) Electron-dense aggregates of mob4geh homozygotes often showed a lattice structure (arrowhead) and (D) fragmented sarcomeres and widened Z-disks (double-arrowhead) were detected as well. (E) At 3 dpf, Gomori trichrome staining revealed subsarcolemmal dark blue structures within mob4geh homozygotes but not siblings (n = 6 per genotype). Boxed areas are shown in higher magnification. (F) GFP fluorescence of transgenic ACTA1-GFP showed a striated pattern in 3-dpf-old siblings and a uniform pattern in mob4geh homozygotes. Expression of ACTA1D286G-GFP led to rod-shaped structures in siblings and exclusively amorphic aggregates within mob4geh homozygotes (n = 6 per genotype). Scale bar sizes are indicated.

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