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FIGURE 6
Fibroblast growth factor 21 up-regulates autophagy through AMPK-mTOR signaling pathway. Samples were collected from three groups at 0, 24, and 48 hpa. (A) Western blotting showing levels of AMPK, p-AMPK, mTOR, p-mTOR, LC3B, and P62, corrected for β-actin as internal control. (B) Quantification of immunohybridization signal of AMPK and p-AMPK by grayscale values. (C) Quantification of immunohybridization signal of mTOR and p-mTOR by grayscale values. (D) Quantification of immunohybridization signal of LC3B and P62 by grayscale values. DMSO group: without Mtz treatment, DMSO was applied. Mtz group: after Mtz treatment, grow naturally. Mtz+FGF21 group: after Mtz treatment, treated with FGF21 standard protein. Significance: *p < 0.05, **p< 0.01, and ***p< 0.001 vs. DMSO group. Data are expressed as mean ± SEM (n = 40 per group, repeat 3 times for each test).