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Figure 1—figure supplement 2. Determining biotin toxicity and tolerance in zebrafish embryos.

(A) The fish embryos were incubated with biotin solution at indicated concentration from 1 to 5 dpf. Fifty WT embryos were used for each treatment group. Dead embryos were removed and counted each day. The statistics were performed using Log-rank (Mantel-Cox) test; ns denotes not significant. (B) representative images showing the fish embryo morphology at 3 dpf with or without biotin (600 µM) incubation.

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