Figure 1—figure supplement 1.

Figure 1—figure supplement 1.

(A) Streptavidin-HRP blot showing biotinylated proteins in BioID (BirA*) transgenic zebrafish embryos. Zebrafish embryos were incubated with 50 µM biotin for 18 hr prior to protein extraction and western blotting. (B, C and D) Streptavidin-HRP blots showing biotinylated proteins in zebrafish embryos with (B) increased biotin concentration, (C) increased biotin incubation time and (D) addition of DMSO. (E and F) Streptavidin-HRP blots showing biotinylated proteins in BioID2 transgenic zebrafish embryos under (E) standard incubation temperature (28°C) and (F) elevated temperature (32.5°C) during biotin incubation. Each sample is a pool of 30 embryos at 3 dpf. The two prominent protein bands present in all samples were endogenous biotinylated proteins.