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Figure Caption

Figure 5—figure supplement 1. <italic>alk5</italic> endothelial-specific overexpression is sufficient to restore OFT wall formation and function in <italic>alk5</italic> mutants.

(A–A’’) Ventral view of 120 hpf Tg(fli1a:alk5b-mScarlet) alk5+/+ larvae, showing the restricted expression of the transgene in the ECs. Boxed area is shown in A’ and A’’; dotted lines outline the OFT; arrowheads, SMCs. (B, C) Brightfield images of 9 dpf alk5+/+ (B) and alk5-/- animals carrying the EC-specific rescue transgene (Tg,C) (11/108 alk5 mutants analyzed inflated their swim bladder). Black arrowheads, swim bladder; pink arrowhead, deformation of the head. (D) Percentage of SMCs surrounded by Eln2 immunostaining (per sagittal plane) at 75 hpf. (E) Percentage of EdU+ SMCs in 72 hpf alk5+/+, alk5-/-, and Tg(fli1a:alk5b-mScarlet) alk5-/- animals. (D, E) Means ± SD; p value from Kruskal-Wallis tests, compared with the first column (alk5+/+). Scale bars: (A) 50 μm; (A’, A’’) 20 μm; (B, C) 200 μm.

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