IMAGE

Fig. 3

ID
ZDB-IMAGE-180822-18
Antibodies
Source
Figures for Lin et al., 2018
Image
Figure Caption

Fig. 3

Ca2+-sensing receptor (CaSR) protein expression in neuromast hair cell of CaSR morpholino oligonucleotide (MO) knockdown larvae. Immunocytochemical staining with CaSR antibodies was conducted in 4-dpf wild-type larvae (A,B), control MO-injected larvae (control-MO; C,D), and CaSR-MO-injected larvae (CaSR-MO; E–H). CaSR signals (green) were detected in the apical (arrows; A,C) and basolateral (arrows; B,D) membranes of hair cells in wild-type and control-MO larvae. In CaSR-MOs, the CaSR signal was weak in the apical and basolateral membranes (E–H). Scanning electron microscopy images revealed the morphologies of L1 neuromat hair bundles in 4-dpf control-MOs and CaSR-MOs (arrowheads; I,K,M). Immunocytochemical staining with S100 antibodies was conducted in 4-dpf control-MOs and CaSR-MOs (J,L,N). The hair cell numbers of L1 neuromasts in the posterior lateral lines were determined (O). Data are presented as mean ± SE. No significant differences were identified (one-way ANOVA and Tukey’s comparison, p < 0.05). Numbers in parentheses are the numbers of neuromasts. One L1 neuromast per larva was examined.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Physiol.