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Fig. 7
RA signaling regulates level of prune2 transcripts in the zebrafish embryos. Embryos at 29 hpf were treated with RA (Sigma, 0.1 μM in DMSO) or DEAB (Aldrich, 10 μM in DMSO) for 3 h. The treated embryos were fixed at 35 hpf for WISH analysis. prune2 transcripts were elevated in the RA treated embryos (B, B′) but reduced in the DEAB treated embryos (C, C′), which were further confirmed by qPCR (D). A, B, C; lateral view anterior to left. A′, B′, C'; dorsal view anterior to left. All scale bars in red indicate 50 μm.
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Reprinted from Gene expression patterns : GEP, 23-24, Anuppalle, M., Maddirevula, S., Kumar, A., Huh, T.L., Choe, J., Rhee, M., Expression patterns of prune2 is regulated by Notch and retinoic acid signaling pathways in the zebrafish embryogenesis, 45-51, Copyright (2017) with permission from Elsevier. Full text @ Gene Expr. Patterns