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Fig. 4
Cirhin-deficient larvae have dose-dependent defects in hepatobiliary function.
(A) Brightfield (left) and fluorescent (right) images of two cirh1a IE14 MO-injected 5 dpf larvae, 2 hours following their ingestion of BODIPY-FL C16 and fluorescent microspheres. Lower larva shows fluorescent lipid accumulation in gallbladder (single arrow and lower inset) indicating normal biliary function, while the other larva has defective biliary function and non-visible gallbladder (double arrow and upper inset), despite normal swallowing of substrate in both fish (fluorescent microspheres in gut, arrowheads). (B) Quantitation of gallbladder fluorescence in control and Cirhin-deficient larvae that had been injected with either the translation-blocking (ATG) or splice-blocking (IE14) MO. *, p<0.05 vs Mismatch MO 1 ng; #, p<0.05 vs Mismatch MO 1.5 ng; **, p<0.05 vs cirh1a ATG MO 0.5 ng; ***, p<0.05 vs cirh1a IE14 MO 0.5 ng.