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Fig. 2
Zebrafish cirh1a is expressed in the developing liver and anterior gastrointestinal tract.
(A-C) Whole-mount in situ hybridization for cirh1a mRNA, showing widespread expression at 20 hpf (A), followed by expression in the developing liver, gallbladder/pancreas, and anterior intestine at 3 dpf (B-C). (D) Close-up image of panel (C). (E) Immunohistochemistry and fluorescent in situ hybridization in 4 dpf Tg(bglob:EGFP) larvae, showing widespread cirh1a liver expression with focal expression in GFP-positive biliary cells (arrowheads). (F) Quantitation of cirh1a expression in GFP-positive biliary cells in 3 dpf and 4 dpf Tg(bglob:EGFP) larvae.