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Fig. 3 RNA in situ expression of mylpfa (green) and smyhc1 (red) in (A) control, (B) pbx2/4-MO, (C) prdm1a-/-, and (D) pbx2/4-MO;prdm1a-/- embryos. Embryos are 14 somites stage, shown in dorsal view, anterior towards the left. Somite numbers 2–7 are shown for each embryo. Yellow lines indicate where optical sections are taken through the x and y planes (lower and right panels, respectively, each image). Dorsal–ventral (D–V) axes (z axis in xy view) are indicated. Arrowheads point to lateral domain of mylpfa expression, absent or reduced in pbx2/4-MO and pbx2/4-MO;prdm1a-/- (asterisk) embryos. smyhc1-overlapping mylpfa expression in adaxial cells is present in pbx2/4-MO;prdm1a-/- embryos. Scale bar: 50 μm.