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Fig. 6 The N-Terminal Coiled-Coil Domain Suppresses BNip1 Proapoptotic Activity(A) BNip1b has four distinct domains: a coiled-coil domain, a BH3 domain, a SNARE domain, and a TM domain. Configuration of deletion constructs (BNip1b-ΔTM, BNip1b-ΔSNARE, BNip1b-Δcc, BNip1b-Δcc-BH3(L114A), and BNip1b-Δcc-ΔBH3). (B) Activation of caspase 3 at 7 hpf (upper rows) and morphologies at 8 hpf (lower rows) in embryos injected with mRNA encoding BNip1b, BNip1b-ΔTM, BNip1b-ΔSNARE, BNip1b-Δcc, and BNip1b-Δcc-ΔBH3. Severe embryonic death and morphological defects are observed only in BNip1Δcc-expressing embryos. (C) Percentages of 8 hpf embryos classified as classes I–IV in the injection experiments shown in (B) (lower panels), as well as BNip1b-Δcc-BH3(L114A). (D) Percentage of class I/II embryos. Green and black bars indicate the means and SDs, respectively. The numbers of injection experiments and t test p values are shown in Table S2. **p < 0.01. Scale bars, 200 μm (B, upper panels) and 600 μm (B, lower panels). See also Figure S7.
Reprinted from Developmental Cell, 25(4), Nishiwaki, Y., Yoshizawa, A., Kojima, Y., Oguri, E., Nakamura, S., Suzuki, S., Yuasa-Kawada, J., Kinoshita-Kawada, M., Mochizuki, T., and Masai, I., The BH3-Only SNARE BNip1 Mediates Photoreceptor Apoptosis in Response to Vesicular Fusion Defects, 374-387, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell