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Fig. 3 gata5 is a target of miR-92. (A) The GFP reporter fused to the 32UTR of gata5. Base pairing between miR-92 and two miRNA recognition elements (MREs) is shown. (B-G) Single-cell zebrafish embryos were injected with mRNAs encoding GFP reporters and fluorescence was monitored at 1 dpf. Embryos were injected with reporters containing either the full-length gata5 32UTR (full) or a construct in which both MREs were deleted (D12). MO refers to miR-92 MO. (H) Western blot of lysates from embryos injected as above were performed with antibodies against GFP or α-tubulin as a loading control. (I) Quantification of relative GFP expression. Error bars represent s.e.m. *, P<0.01, between indicated injection and control embryos (n>3); Student′s t-test.