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Fig. 4 Expression of par1 during zebrafish embryogenesis. A–V: Whole-mount in situ hybridization was performed on embryos at various stages: eight-cell (A); sphere (B); gastrula (C–E); 10-somite (F–H, arrows indicate posterior and ventral mesoderm); 1 days postfertilization (dpf; I–N; I: lateral view of a embryo showing par1 expression in the heart, posterior cardinal vein [PCV], cardinal vein [CV], intermediate cell mass of mesoderm [ICM]; J: high-magnification image of anterior region of I; K: dorsal view of the anterior region of the embryo showing the par1 expression in common cardinal vein [CCV]; L: high-magnification image of the trunk region of I, location of the notochord (nc) is indicated; M,N: sagittal and transverse sections of the trunk area indicated by the black line shown in I); day 2 (O–S; O: Lateral view showing par1 expression in the heart, branchial arches, caudal hematopoietic tissue [CHT]; P: dorsal view of the anterior region of O at high magnification; Q,R: lateral view of the trunk region of O at high magnification; S: transverse section at the trunk region showing par1 expression in notochord [nc], vein, pronephric duct [pd], and gut); 3 dpf (T–U; T: lateral view showing par1 expression in the heart, pharynx, CHT; U: dorsal view of the anterior region at high magnification; par1 expression in pancreas is indicated). Most embryos are shown in lateral view, with the animal pole up. Exceptions include G (dorsal view), H (dorsal–posterior view), and K, P, U (dorsal view). Scale bars = 100 μm.