ZFIN Image: Hart et al., 2009, Fig. 2

Image

Figure Caption

Fig. 2 Taf3 is selectively recruited to the promoter of the Trf3 target gene mespa in vivo and is required for mespa expression. A: RT-PCR analysis monitoring expression of mespa in control and taf3 MO-treated embryos at 6 hpf. B: ChIP analysis monitoring occupancy of Trf3, Tbp, and Taf3 at the mespa and actin promoters in embryos at 6 hpf. Control ChIP experiments were performed in the absence of antibody (No ab). A schematic representation of the mespa promoter showing the location of the primer pairs used for ChIP analysis is shown (bottom). C: Whole-mount in situ hybridization with riboprobes to gata1 and hbbe1 (28 hpf) in embryos injected with a control MO or taf3 MO, or in taf3 MO-treated embryos injected with a mespa mRNA.

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Acknowledgments

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