ZFIN Image: Steffen et al., 2007, Fig. 7

Image

Figure Caption

Fig. 7 The ruz dystrophy shows increased calpain-3 and loss of sarcomeric MuRF2 expression. (A) Western blot analysis of 6.5 dpf wild-type and ruz mutants shows a mild increase in calpain-3. Actin expression is used as a loading control. (B, C) Indirect immunofluorescence using MuRF2 antibodies (red) was performed on longitudinally sectioned skeletal muscle of 6.5 dpf fish. Nuclei were stained with DAPI (blue). (B) In wild-type fish, MuRF2 is localized to both the sarcomere (striations) and nucleus (arrow). (C) MuRF2 is lost at the sarcomere in ruz mutants but no significant increase in nuclear localization is detected.

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Phenotype details

Acknowledgments

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Reprinted from Developmental Biology, 309(2), Steffen, L.S., Guyon, J.R., Vogel, E.D., Howell, M.H., Zhou, Y., Weber, G.J., Zon, L.I., and Kunkel, L.M., The zebrafish runzel muscular dystrophy is linked to the titin gene, 180-192, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.