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Fig. 5 Following knockdown of Lmx1b.1 and Lmx1b.2, IsO genes are initiated normally, but some are not maintained. Lateral views of anterior CNS, with dorsal upwards and anterior leftwards. Embryos were injected with B1B2-MO (A,C,E,G) or BX-MO (B,D,F,H). wnt1 is initiated normally at the 10-somite stage. (A,B), but fades at the MMR by 15-18 somites (C,D). pax8 expression fades by 15-18 somites (E,F), and fgf8 fades by 19-22 somites, with partial dorsal retention (G,H). Red arrowheads indicate the MMR.
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