CRISPR

CRISPR2-tmem216

ID
ZDB-CRISPR-210312-2
Name
CRISPR2-tmem216
Previous Names
None
Target
Sequence
5' - CCCACAAGATAATCTGATATTGG - 3'
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
None
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
zf3394 tmem216
zf3395 tmem216
Expression
Gene expression in Wild Types + CRISPR2-tmem216
No data available
Phenotype
Phenotype resulting from CRISPR2-tmem216
No data available
Phenotype of all Fish created by or utilizing CRISPR2-tmem216
Phenotype Fish Conditions Figures
retinal cone cell cone photoreceptor outer segment ab2-gnat2 labeling absent, abnormal tmem216zf3394/zf3394 (AB/TU) standard conditions Figure 4. with image from Liu et al., 2020
photoreceptor cell filamentous actin disorganized, abnormal tmem216zf3394/zf3394 (AB/TU) standard conditions Figure 6. with image from Liu et al., 2020
whole organism semi-viable, abnormal tmem216zf3394/zf3394 (AB/TU) standard conditions Table 1 from Liu et al., 2020
retinal outer nuclear layer apoptotic process increased process quality, abnormal tmem216zf3394/zf3394 (AB/TU) standard conditions Figure 7. with image from Liu et al., 2020
whole organism dead, abnormal tmem216zf3394/zf3394 (AB/TU) standard conditions Table 1 from Liu et al., 2020
long double cone cell cone photoreceptor outer segment ab3-rho labeling absent, abnormal tmem216zf3394/zf3394 (AB/TU) standard conditions Figure 4. with image from Liu et al., 2020
retinal cone cell cell body ab2-gnat2 labeling mislocalised, abnormal tmem216zf3394/zf3394 (AB/TU) standard conditions Figure 4. with image from Liu et al., 2020
retinal outer nuclear layer axoneme decreased amount, abnormal tmem216zf3394/zf3394 (AB/TU) standard conditions Figure 8. with image from Liu et al., 2020
long double cone cell cell body ab3-rho labeling mislocalised, abnormal tmem216zf3394/zf3394 (AB/TU) standard conditions Figure 4. with image from Liu et al., 2020
retinal cone cell ab2-gnat2 labeling decreased distribution, abnormal tmem216zf3394/zf3394 (AB/TU) standard conditions Figure 3. with image from Liu et al., 2020
whole organism viability, abnormal tmem216zf3394/zf3394 (AB/TU) standard conditions Table 1 from Liu et al., 2020
retinal outer nuclear layer axoneme ab1-tuba labeling decreased distribution, abnormal tmem216zf3394/zf3394 (AB/TU) standard conditions Figure 8. with image from Liu et al., 2020
retinal outer nuclear layer axoneme area density, abnormal tmem216zf3394/zf3394 (AB/TU) standard conditions Figure 8. with image from Liu et al., 2020
long double cone cell ab3-rho labeling decreased distribution, abnormal tmem216zf3394/zf3394 (AB/TU) standard conditions Figure 3. with image from Liu et al., 2020
retinal rod cell cell body ab5-rho labeling mislocalised, abnormal tmem216zf3394/zf3394 (AB/TU) standard conditions Figure 6. with image from Liu et al., 2020
retinal rod cell rod photoreceptor outer segment ab5-rho labeling decreased distribution, abnormal tmem216zf3394/zf3394 (AB/TU) standard conditions Figure 5. with image from Liu et al., 2020
retinal outer nuclear layer axoneme decreased length, abnormal tmem216zf3394/zf3394 (AB/TU) standard conditions Figure 8. with image from Liu et al., 2020
eye photoreceptor cell vacuole increased amount, abnormal tmem216zf3395/zf3395 (AB/TU) standard conditions Figure 9. with image from Liu et al., 2020
retinal cone cell cone photoreceptor outer segment ab2-gnat2 labeling absent, abnormal tmem216zf3395/zf3395 (AB/TU) standard conditions Figure 4. with image from Liu et al., 2020
whole organism semi-viable, abnormal tmem216zf3395/zf3395 (AB/TU) standard conditions Table 1 from Liu et al., 2020
retinal cone cell cell body ab2-gnat2 labeling mislocalised, abnormal tmem216zf3395/zf3395 (AB/TU) standard conditions Figure 4. with image from Liu et al., 2020
retinal outer nuclear layer apoptotic process increased process quality, abnormal tmem216zf3395/zf3395 (AB/TU) standard conditions Figure 7. with image from Liu et al., 2020
whole organism dead, abnormal tmem216zf3395/zf3395 (AB/TU) standard conditions Table 1 from Liu et al., 2020
long double cone cell cone photoreceptor outer segment ab3-rho labeling absent, abnormal tmem216zf3395/zf3395 (AB/TU) standard conditions Figure 4. with image from Liu et al., 2020
head ab2-gnat2 labeling decreased amount, abnormal tmem216zf3395/zf3395 (AB/TU) standard conditions Figure 3. with image from Liu et al., 2020
eye photoreceptor cell photoreceptor outer segment morphology, abnormal tmem216zf3395/zf3395 (AB/TU) standard conditions Figure 9. with image from Liu et al., 2020
retinal cone cell ab2-gnat2 labeling decreased distribution, abnormal tmem216zf3395/zf3395 (AB/TU) standard conditions Figure 3. with image from Liu et al., 2020
long double cone cell cell body ab3-rho labeling mislocalised, abnormal tmem216zf3395/zf3395 (AB/TU) standard conditions Figure 4. with image from Liu et al., 2020
whole organism viability, abnormal tmem216zf3395/zf3395 (AB/TU) standard conditions Table 1 from Liu et al., 2020
eye photoreceptor cell photoreceptor disc membrane shortened, abnormal tmem216zf3395/zf3395 (AB/TU) standard conditions Figure 9. with image from Liu et al., 2020
long double cone cell ab3-rho labeling decreased distribution, abnormal tmem216zf3395/zf3395 (AB/TU) standard conditions Figure 3. with image from Liu et al., 2020
retinal rod cell rod photoreceptor outer segment ab5-rho labeling decreased distribution, abnormal tmem216zf3395/zf3395 (AB/TU) standard conditions Figure 5. with image from Liu et al., 2020
Citations