CRISPR

CRISPR3-e2f4

ID
ZDB-CRISPR-201005-1
Name
CRISPR3-e2f4
Previous Names
None
Target
Sequence
5' - AGATCTCAAGTTGGAACTGG - 3'
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
None
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
ouc2022 e2f4
Expression
Gene expression in Wild Types + CRISPR3-e2f4
No data available
Phenotype
Phenotype resulting from CRISPR3-e2f4
No data available
Phenotype of all Fish created by or utilizing CRISPR3-e2f4
Phenotype Fish Conditions Figures
pronephric tubule single ciliated epithelial cell increased amount, abnormal e2f4ouc2022/ouc2022 standard conditions Fig 4 with image from Xie et al., 2020
pronephric tubule multi-ciliated epithelial cell absent, abnormal e2f4ouc2022/ouc2022 standard conditions Fig 4 with image from Xie et al., 2020
pronephric proximal convoluted tubule multi-ciliated epithelial cell absent, abnormal e2f4ouc2022/ouc2022 standard conditions Fig 4 with image from Xie et al., 2020
pronephric tubule multi-ciliated epithelial cell decreased amount, abnormal e2f4ouc2022/ouc2022 standard conditions Fig 4 with image from Xie et al., 2020
pronephric tubule single ciliated epithelial cell increased amount, exacerbated e2f4ouc2022/ouc2022; e2f5ouc2019/ouc2019 standard conditions Fig 4 with image from Xie et al., 2020
olfactory pit multi-ciliated epithelial cell absent, abnormal e2f4ouc2022/ouc2022; e2f5ouc2019/ouc2019 standard conditions Fig. S5 from Xie et al., 2020
pronephric tubule multi-ciliated epithelial cell absent, abnormal e2f4ouc2022/ouc2022; e2f5ouc2019/ouc2019 standard conditions Fig 4 with image from Xie et al., 2020
pronephric proximal convoluted tubule multi-ciliated epithelial cell absent, abnormal e2f4ouc2022/ouc2022; e2f5ouc2019/ouc2019 standard conditions Fig 4 with image from Xie et al., 2020
maintenance of ciliary planar beating movement pattern disrupted, abnormal e2f4ouc2022/ouc2022; e2f5ouc2019/ouc2019 standard conditions Fig 4 with image from Xie et al., 2020
pronephric tubule increased diameter, abnormal e2f4ouc2022/ouc2022; e2f5ouc2019/ouc2019 standard conditions Fig 4 with image from Xie et al., 2020
pronephric proximal straight tubule multi-ciliated epithelial cell absent, exacerbated e2f4ouc2022/ouc2022; e2f5ouc2019/ouc2019 standard conditions Fig 4 with image from Xie et al., 2020
Citations