CRISPR

CRISPR1-sucla2

ID

ZDB-CRISPR-200827-2

Name

CRISPR1-sucla2

Previous Names

None

Target

sucla2 (1)

Sequence

5' - GGCAAAGGCACTTTTGA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
nei010	sucla2

Expression

Gene expression in Wild Types + CRISPR1-sucla2

No data available

Phenotype

Phenotype resulting from CRISPR1-sucla2

No data available

Phenotype of all Fish created by or utilizing CRISPR1-sucla2

Phenotype	Fish	Conditions	Figures
aerobic respiration decreased occurrence, abnormal	sucla2^{nei010/nei010}	standard conditions	Fig. 5 from Gut et al., 2020
whole organism sucla2 expression decreased amount, abnormal	sucla2^{nei010/nei010}	standard conditions	Fig. 4 from Gut et al., 2020
whole organism viability, abnormal	sucla2^{nei010/nei010}	standard conditions	Fig. 5 from Gut et al., 2020
aerobic respiration decreased occurrence, abnormal	sucla2^{nei010/nei010}	chemical treatment by environment: carbonyl cyanide p-trifluoromethoxyphenylhydrazone	Fig. 5 from Gut et al., 2020
whole organism mitochondrial chromosome decreased amount, abnormal	sucla2^{nei010/nei010}	standard conditions	Fig. 5 from Gut et al., 2020
whole organism sucla2 expression absent, abnormal	sucla2^{nei010/nei010}	standard conditions	Fig. 4 from Gut et al., 2020
whole organism viability, abnormal	sucla2^{nei010/nei010}; nei005Tg/nei005Tg	standard conditions	Fig. 5 from Gut et al., 2020
whole organism sucla2 expression absent, abnormal	sucla2^{nei010/nei010}; nei005Tg/nei005Tg	standard conditions	Fig. 4 from Gut et al., 2020
whole organism mitochondrial chromosome decreased amount, abnormal	sucla2^{nei010/nei010}; nei005Tg/nei005Tg	standard conditions	Fig. 5 from Gut et al., 2020
aerobic respiration normal occurrence, ameliorated	sucla2^{nei010/nei010}; nei005Tg/nei005Tg	chemical treatment by environment: carbonyl cyanide p-trifluoromethoxyphenylhydrazone	Fig. 5 from Gut et al., 2020
aerobic respiration decreased occurrence, abnormal	sucla2^{nei010/nei010}; nei005Tg/nei005Tg	standard conditions	Fig. 5 from Gut et al., 2020
whole organism sucla2 expression absent, abnormal	sirt5^{nei004/nei004}; sucla2^{nei010/nei010}	standard conditions	Fig. 4 from Gut et al., 2020

Citations